Conditioned taste aversion was carried out in the manner described by Blednov et al. (2003). After recovery from LPS pretreatment, mice were adapted to a water-restriction schedule (2 h of water per day) over a 7-d period. At 48-h intervals over the next 10 d (days 1, 3, 5, 7, 9, and 11), all mice received 1-h access to a solution of saccharin (0.15% w/v sodium saccharin in tap water). Immediately after 1-h access to saccharin, the mice received injections of saline or ethanol (2.5 g/kg) (days 1, 3, 5, 7, and 9). Mice also received 30-min access to tap water 5 h after each saccharin access period to prevent possible dehydration, and this was followed by injection of ethanol (2.5 g/kg, i.p.) (days 1, 3, 5, 7, and 9). On intervening days, the mice had 2 h of continuous access to water at standard times in the morning (days 2, 4, 6, 8, and 10).
