As the Pearson’s correlation revealed only the potential physical association between gene expression levels rather than the direct analysis of the interplay between two proteins, we investigated the potential palmitoylation with the amino acid sequences of the two proteins. INCENP can bind to the oncogenes survivin and borealin through its N-terminal domain (INCENP_N) form a trimer to exert its cancer-promoting function (Klein et al., 2006; Vader et al., 2006). The conserved motif encompassing amino acids 32–44 has been proven to be essential for targeting INCENP to centromeres during mitosis, and the first 26 amino acids in the protein sequence of INCENP has also been reported to facilitate this functional targeting centromeres (Ainsztein et al., 1998). However, effects of the deletion of the INCENP protein structure have not been excluded. CSS-Palm 4.0 is a software for in silico predicting palmitoylation sites based on proteomic analysis, including a fourth-generation Group-based Prediction System (GPS) algorithm (Ren et al., 2008). The latest training data set contains 583 palmitoylation sites from 277 proteins, experimentally proved and reported in scientific literature. Particle Swarm Optimize (PSO) was
