The hiPSCs were incubated with TrypLE Select for 5 min, then dissociated into single cells by pipetting. The cells were plated at a density of 10 000 cells in an uncoated 24-well plate, containing neural culture medium supplemented with human leukemia inhibitory factor and basic fibroblast growth factor. The cells were then cultured in an atmosphere containing 4% oxygen and 5% carbon dioxide, for 5 days.
