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Chunk #5 — Results — Conditional Deletion of Zfhx1b in the VZ or the SVZ of the Subpallium Using Nkx2.1-Cre or DlxI12b-Cre

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Dlx1&2-dependent expression of Zfhx1b (Sip1, Zeb2) regulates the fate switch between cortical and striatal interneurons.
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Zfhx1b prenatal expression has been noted in migrating cortical interneurons and the subpallial telencephalon (Batista-Brito et al., 2008; Seuntjens et al., 2003). We found that Zfhx1b RNA is expressed in E12.5 MGE-derived cells that are tangentially migrating through the LGE and into the cortex by performing fluorescent in situ hybridization (FISH) on a brain in which MGE-derived cells expressed EGFP (expressed due to Nkx2.1-Cre induced recombination of the CAG:CAT-EGFP Cre reporter allele) (Figures S1 A-A”).