Female WT, KO, and KI mice were initially fed the control liquid dextrose diet (Bio-Serv, Frenchtown, NJ) for 3 days to acclimate them to the liquid diet. Afterward, the mice were fed either the liquid ethanol diet (Bio-Serv, Frenchtown, NJ) or the control liquid dextrose diet, as described by Lieber and DeCarli [24] for 3 weeks. The content of ethanol was gradually increased every 3–4 days from 10% (1.77% [vol/vol]) of total calories to 20% (3.54% [vol/vol]), 25% (4.42% [vol/vol]), 30% (5.31% [vol/vol]), and finally 35% of total calories (6.2% [vol/vol]). The control mice were pair-fed the control dextrose diet on an isoenergetic basis. The ethanol-fed mice had access to their rations ad libitum, and the conditions of wild-type, knockout, and humanized transgenic mice were comparable. The amount of food consumed by CYP2E1-knockout mice, the knockin mice and the wild-type mice was approximately the same.
