Detailed information is provided in the Supplement 1. Briefly, Homer 1b/c and control mouse IgG (Santa Cruz) antibodies were crosslinked to Dynabeads (Invitrogen) using Bis(Sulfosuccinimidyl) suberate (Thermo Scientific) and incubated with solubilized protein (500 μg). The protein complexes were separated by SDS-PAGE, transferred to nitrocellulose membranes which were blocked and then probed with either 1 µg/ml Homer 1b/c or Dynamin 3 (Abcam) antibodies overnight at 4°C. The blots were developed and analyzed as described above.
