Because RRBS is a sequence-based assay with single-base resolution, we were able to identify CpGs with allele-specific methylation consistent with genomic imprinting, and determined that these loci exhibit aberrant methylation in cancer cell lines (K. Varley et al. Personal Communication). Furthermore, we detected reproducible cytosine methylation outside CpG dinucleotides in adult tissues45, providing further support that this non-canonical methylation event may play important roles in human biology (K. Varley et al. Personal Communication).
