Chunk #9 — 2. Materials and Methods — 2.3 Behavioral Testing

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Inhibition of endocannabinoid catabolic enzymes elicits anxiolytic-like effects in the marble burying assay.

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Locomotor activity was simultaneously captured during the test, using Unibrain Fire-I digital cameras and analyzed in real time using ANY-maze software (Stoelting, Kiel, WI). Immobility was defined as a lack of movement for 1250 ms or longer, and was analyzed in 1 min bins.