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Chunk #24 — General Methods — Sucrose and Drug preparation procedures

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Adolescent but not adult rats exhibit ethanol-mediated appetitive second-order conditioning.
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Ethanol doses (0.5 and 2.0 g/kg) were achieved by intragastrically administering 0.015 ml of a 4.2 or 16.8 % v/v ethanol solution per gram of body weight (190-proof Ethanol, Pharmaco, Brookfield; vehicle: tap water). The administration was conducted in less than 10 sec by gently introducing a 12-cm section of PE polyethylene tubing (Clay-Adams) into the animal’s oral cavity. The tubing (diameter: 10” or 50”, for adolescents and adults, respectively) was connected to a syringe (5 or 12 cc, adolescents and adults, respectively) mounted with a 27 or 25 G ½ needle (Becton Dickinson & Co., Rutheford, N.J). About 6 cm of tubing was guided into the subjects’ stomach prior to the delivery of EtOH. Sucrose (Sigma-Aldrich, St. Louis, MO) was prepared daily employing distilled water as a vehicle.