To assess the reproducibility of our protocol among multiple PSC lines, we transduced another three independent iPS cell lines and quantified intrinsic membrane properties, including the frequency and amplitude of spontaneous IPSCs, of the AMD-induced neurons. All these parameters were statistically indistinguishable (one-way ANOVA, P > 0.05) among iN cells derived from different cell lines (Supplementary Fig. 2). We conclude that the combined expression of Ascl1, Myt1l and Dlx2 induces the differentiation of human PSCs into a virtually homogenous population of GABAergic neurons with high yield and reproducibility.
