To examine the effects of acute alcohol exposure on the amplitude of GABA-mediated currents, responses were evoked by pressure application of 50 μM GABA in mature (20–33 week old) neurons derived from CTLs and ADs (Figure 2A). These responses could be consistently evoked every 30 sec over a 35-min recording period (Figure 2B). The acute effect of alcohol was examined in a total of 88 neurons derived from 6 CTL and 7 AD lines (average of ≈7 cells per subject). There was no significant effect of 15-minute bath perfusion of 50 mM alcohol on the amplitude of GABA-evoked response (p = 0.81), and there was no significant interaction between alcohol treatment and donor status (p = 0.48) (Figure 2C).
