Single-cell kill assays were conducted by plating individual iNPCs onto 96-well plates as single cells before differentiation. Differentiation into neuronal lineages was performed as described above in the presence of the indicated chemicals or the respective DMSO controls. Cell survival was estimated with trypan blue after differentiation over 28 days. Each condition was done in 24 replicates with a minimum of two independent times (n⩾48).
