Student's t-test (two-sided) was used for analysis of TH-positive cells as percent of total (Figure 2D), mitochondria length (aspect ratio) as well as mitochondrial interconnectivity (form factor) (Figure 3C). One-way ANOVA with Dunnet's post-hoc test was used for quantifications of percent of mitochondrial motility (Figure 3D). p values less than 0.05 were considered significant. Statistical calculations were performed with GraphPad Prism Software, Version 5.0 (http://www.graphpad.com/scientific-software/prism/). All data shown is representative of experiments from at least 3 separate differentiated culture sets.
