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Chunk #1 — Methods and Materials — Animals

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The Creb1 coactivator Crtc1 is required for energy balance and fertility.
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Male C57BL/6J, BKS.Cg-m +/+ Leprdb/J, and B6.V LepOb mice were obtained from Jackson Laboratories. All mice were allowed to adapt to their environment for at least 1 week prior to use in studies.