Chunk #10 — MATERIALS AND METHODS — RNA sequencing and analysis

Source

Gene Expression Changes in Glutamate and GABA-A Receptors, Neuropeptides, Ion Channels, and Cholesterol Synthesis in the Periaqueductal Gray Following Binge-Like Alcohol Drinking by Adolescent Alcohol-Preferring (P) Rats.

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Qiagen Ingenuity Pathway Analysis (IPA) was performed on the genes significant at FDR ≤ 0.05. Pathways that contained fewer than 5 differentially expressed genes were dropped. Pathways with the same list of genes were collapsed (e.g. multiple cholesterol synthesis pathways).