Chunk #17 — MATERIALS AND METHODS — Transplantation into the 6-Hydroxydopamine Rats, Behavioral and Histological Analyses

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Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions.

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For rational behavior analysis, the animals were given 2.5 mg/kg d-amphetamine intraperitoneally, and their rotational behavior was monitored over a 90-minute period using the rotameter system (TSE) (Bad Homburg, Germany). Rotation toward the lesion (ipsilateral) was scored as positive and net rotational asymmetry score were expressed as full body turns per minute.