We ordered custom FISH probes from ACD to validate MSN subtypes as follows: DRD1 (ACD #549041, a 20ZZ probe targeting 1335-2279 of NM_001206975.1), DRD2 (ACD #549031-C2, a 20ZZ probe targeting 232-1470 of XM_001085571.3), RXFP1 (ACD #801121-C2, a 20ZZ probe targeting 1508-2592 of XM_001096574.4), CPNE4 (ACD #801111-C3, a 20ZZ probe targeting of 2-943 of XM_028843706.1), KCNIP1 (ACD #889143-C3, a 20ZZ probe targeting 283-1626 of XM_015141392.2), KCNT1 (ACD #881571-C3, a 20ZZ probe targeting 964-1906 of XM_015116381.2), BACH2 (ACD #898961-C3, a 20ZZ probe targeting 927-2260 of XM_028847343.1), KHDRBS3 (ACD #881591-C3, a 20ZZ probe targeting 494-1493 of XM_028852934.1), STXBP6 (ACD #881611-C2, a 20ZZ probe targeting 2-1077 of NM_001260925.1), SEMA3E (ACD #879971-C2, a 20ZZ probe targeting 905-1889 of XM_028846126.1), GDA (ACD #881601-C2, a 20ZZ probe targeting 355-1373 of XM_015117899.2), GREB1L (ACD #898991-C3, a 20ZZ probe targeting 784-1732 of XM_015121640.2), ARHGAP6 (ACD #898981-C3, a 20ZZ probe targeting 2516-3463 of XM_001094565.3), TAC3 (ACD #520901-C3, a 17 ZZ design and targets 2- 768 bp of XM_001115535.2), and OPRK1 (ACD #518931, a 20 ZZ probe and targets 91–1392 bp of NM_001321097.1).
