H89, a potent PKA inhibitor, had no effect on control γ power, but reduced the ethanol-induced suppression of γ oscillations (example in Figure 3A). On average, H89 (10 μM) caused 2.8 ± 3.6% change in γ power (P > 0.05 vs. baseline, n = 10, Figure 3B). Subsequent ethanol (50 mM) caused a 13.4 ± 4.9% decrease (P < 0.05 vs. H89 baseline, n = 10) in γ power (Figures 3A,B), which was significantly less than that of 50 mM ethanol alone (Student t-test, P < 0.05). In the presence of H89 the 100 mM ethanol-induced reduction of γ power was 33.4 ± 4.3% (P < 0.01 vs. H89 baseline, n = 10), which was smaller than that of 100 mM ethanol alone (Student t-test, P < 0.05, Figure 3B). These results indicate that PKA is involved in the mechanism underlying ethanol-induced suppression of γ oscillations.
