This novel experimental validation pipeline is significantly more effective than unbiased transcriptome profiling through RNA sequencing, which is becoming the norm. Exon–exon junctions specific to a single GENCODE annotated transcript are five times more likely to be corroborated with our targeted approach than with extensive large human transcriptome profiling such as the HBM and ENCODE RNA-seq (validation rates of 82% and 16%, respectively), as random sampling of RNA molecules leads to poor assessment of low expressed transcripts. It should be noted that the RNA-seq samples were deeply sequenced with the resulting data sets and contained a total of 4.99 and 5.55 billion sequence reads, respectively.
