of neurons. Interestingly, quantitative measurements of spontaneous neurotransmitter release revealed a significant decrease in the frequency of the spontaneous ESPCs (sEPSCs) in the SCZD neural network (Figure 7H; 0.76 ± 0.14 Hz, n = 42, p < 0.003) compared to the control neurons (3.0 ± 0.70 Hz, n = 40) as well as a significant reduction in the amplitude of the sEPSCs (Figure 7H; SCZD, 13.66 ± 1.05 pA; control, 17.66 ± 1.60 pA; p < 0.05). Thus, consistent with a previous study (Brennand et al., 2011), our data suggested that delayed conversion of SCZD NPCs into hippocampal granule neurons resulted in the attenuated functional network connectivity and activity levels observed in the SCZD hiPSC-derived neuronal culture.
