Chunk #55 — Methods — Flow cytometry analysis of Csf1r−/− and PLX-3397-treated animals

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Neurotoxic reactive astrocytes are induced by activated microglia.

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For P8 Csf1r−/− animals, brains were processed identically except that the myelin depletion step was removed and cells were passed through fine nylon mesh to filter debris (Tetko HC3-20).