Similar to the effect on FoxO1, d-fenfluramine treatment greatly increased the levels of phospho-Ser253-FoxO3a and phospho-Thr32-FoxO3a in the cerebral cortex, hippocampus, and striatum (Figure 1c). The level of phosphorylated FoxO3a after 2-hr d-fenfluramine treatment was significantly higher than saline controls, whereas the level of total FoxO3a was not changed by d-fenflurmaine in the tested brain regions (Figure 1d).
