With regard to the stability of alcohol intake prior to and following deprivation, when alcohol intake prior to alcohol deprivation (mean of 5 days) was compared to alcohol intake during reaccess to alcohol on day 1, there was a significant effect of treatment, F (3, 42) = 5.0, p<0.01, and day F (1, 42) = 21.5, p<0.001, but no treatment X day interaction (Fig. 3A). Dunnett’s multiple comparisons against a single mean (VEH) revealed that, in the VEH-treated group, there was no change in alcohol intake on day 1 of alcohol reaccess when compared to alcohol intake prior to alcohol deprivation (Fig. 3). VAR, in doses of 0.5 and 2.0 mg/kg BW each reduced alcohol intake on day 1 of reaccess when compared to alcohol intake prior to the second deprivation (p<0.01, p<0.001, respectively), indicating a rapid onset of action of VAR (Fig. 3).
