Chunk #22 — METHODS — RNA extraction, cDNA synthesis and quantitative RT-PCR (qPCR) analysis

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A three-dimensional human neural cell culture model of Alzheimer's disease.

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the neuronal and glial marker gene names and PCR product sizes: NCAM1 (neural cell adhesion molecule 1, 174 bp), SYT5 (synaptotagmin V, 171 bp), SLC17A7 (VGLUT1, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter) member 7, 162 bp), GRIN2A (glutamate receptor, ionotropic, N-methyl D-aspartate 2A, 170 bp), EAAT3 (solute carrier family 1, member 1 (neuronal glutamate transporter), 165 bp), ACHE (Acetylcholinesterase, 232 bp), SLC6A4 (solute carrier family 6 member 4 (neurotransmitter transporter, dopamine), 213 bp), GABRA1 (Gamma-aminobutyric acid (GABA) A receptor alpha 1, 165 bp), MAPT (microtubule-associated protein tau, 206), S100β (S100 calcium binding protein, 157 bp), GFAP (glial fibrillary acidic protein, 183 bp), EAAT2 (solute carrier family 1, member 2 (EAAT2, glial), 158 bp), MBP (myelin basic protein, 183 bp) and ACTB (β-actin, 233).