Total RNA was extracted from 60 human liver tissue samples from the University of Washington School of Pharmacy Human Liver Bank as previously described [51], [13]. Genome wide expression analysis was performed using 750 ng of total RNA on the Illumina HumanRef-8 v.2 platform (GPL5060). All liver samples were analyzed with technical replicates that were randomized between processed batches of 24 arrays performed on different days. Raw signal intensity measurements from each sample were processed using the Illumina BeadStudio software v. 2.3.41 using the ‘average’ normalization function. Replicate data from each liver was averaged prior to statistical analysis. All samples and replicates passed quality-control measures.
