even an estimate of zero LFC is not significant, as the large uncertainty of the estimate does not allow us to exclude that the gene may in truth be more than weakly affected by the experimental condition. Note the lack of LFC shrinkage: to find genes with weak differential expression, DESeq2 requires that the LFC shrinkage has been disabled. This is because the zero-centered prior used for LFC shrinkage embodies a prior belief that LFCs tend to be small, and hence is inappropriate here.
