In order to define perturbational classes we first obtained annotations for as many Touchstone perturbagens as possible. For compounds, mechanism of action and gene target annotations were collated from multiple sources (Corsello et al., 2017). For genes, family and pathway annotations were obtained from HGNC as of July 2016. Annotations for both compounds and genes were manually regularized. We next grouped perturbagens by shared annotation to generate candidate classes. For example, all compounds that share the same mechanism of action were assigned to the same class.
