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Chunk #20 — MATERIALS AND METHODS — Genetic analyses

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Replication and extension of genome-wide association study results for obesity in 4923 adults from northern Sweden.
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DNA was extracted at the Medical Biobank in Umeå from peripheral white blood cells. Genomic DNA samples were subsequently diluted to 4 ng/µl. Taqman MGB chemistry (Applied Biosystems, Foster City, CA, USA) was used for all genotyping assays in accordance with the recommended protocol (16). Genotyping success and concordance rates were >94% and >99% for all single nucleotide polymorphisms (SNPs), respectively.