The finding that significant effects on gene expression were not observed at the 24-hr time point in the present study are not in agreement with the results of an operant ethanol self-administration study (Rodd et al., 2008), which reported over 200 significant differences (using a similar FDR and cutoff value) between the ethanol and water groups in the whole ACB. This lack of agreement between the operant study and the present scheduled access drinking study suggests that factors associated with the operant procedure may be having a greater effect than chronic alcohol drinking alone on gene expression within the ACB.
