A full-length mouse NHE9 cDNA was cloned into pcDNA3-EGFP using the following primers GATCATAAGCTTATGGCTGGGCAGCTTCGGTTTACG and ATGCTAGAATTCGTC CATCTGGGGTTGACCCCGAG. HindIII and EcoRI sites were added to facilitate cloning. mNHE9-EGFP was cloned into FuGW –lentiviral vector into the BamH1 site. Stratagene's QuikChange-Site-Directed Mutagenesis Kit was used to make the point mutations.
