Because variant rs11583322 did not work well with the Sequenom genotyping platform, we used the PrimerPicker software [82] to design the assay and followed the protocol described in KASPar SNP Genotyping System manual to run PCR reaction with an ABI GeneAmp PCR System 9700 [83]. Genotypes were accessed using an ABI 7900 HT Fast Real-Time PCR system. Because the genotypes are from linkage families, we used the program UNPHASED [84] to perform a genetic association analysis. Our colleagues in Allison Goate’s lab implemented the above genotyping process. The author did the final analyses of the genotypes. Results are shown in Table 8 .
