Longitudinal brain sections from Sst-Flp;Nos1-Cre;Ai65 animals were perfused and sectioned at 75um thickness then immunolabeled with anti-Caspr (EMD Millipore #MABN69, 1:500 dilution) and anti-RFP (Rockland #600-401-379, 1:1000 dilution). Super resolution images were acquired with GE Healthcare OMX V3 structured illumination microscopy system using: 488 and 593 nm solid state lasers; UPlanS Apochromat 100×1.4 NA objective lens (Olympus); 2 EM-CCD cameras (Cascade II 512, Photometrics). 3D structured illumination images were reconstructed with SoftWoRx® 6.5.2 software. 3D rendering was performed using Imaris (Bitplane) 7.6.5. exported as TIFF, processed in FIJI and assembled in Adobe Illustrator.
