The cycle number imposed little influence on the mean of the flow-value distribution, but given its large dispersion coefficient and that it is a numeric variable as opposed to a factor, its contribution to the overall variance is the second largest from the third flow-cycle on. Interestingly, the chip, and kit made only small contributions to the mean of the flow-value distribution, but had large and substantial influences on the dispersion ( Table 3 ). The newest template kit considered here, the 200 bp One Touch, exhibited significantly less dispersion (variance) in flow-value in contrast to the older kits.