For each biological replicate used for INTACT RNA-seq, flies were pooled such that 2000 fly heads were collected via liquid nitrogen freezing and sieve-separated 24 hr after treatment conditioning. Flies were treated with one of four exposure paradigms (Figure 1A). Biological replicates were performed for air (n = 3), EtOH (n = 3), odors (n = 4), and trained (n = 4) conditions. Odors and trained conditions were performed with reciprocal odor groups (n = 2 ethyl acetate→isoamyl alcohol; n = 2 isoamyl alcohol→ethyl acetate).
