not show equal effects on gene expression, reflecting that CRISPR-Cas9 can display considerable site-to-site variability in its activity, in some cases showing no activity. Nonetheless, for all three SNPs, CRISPRi yielded results that were concordant with the effects observed in the genome-edited cells—decreased CPNE1 expression vis-à-vis the major allele of rs2277862, decreased DOCK7 and increased ANGPTL3 expression vis-à-vis the major allele of rs10889356, and decreased FRK expression vis-à-vis the major allele of rs10872142. It should be noted that while our overall approach was successful in identifying and validating functional variants in the three eQTL loci, it might not have identified all functional variants in the loci, as multiple variants in a locus can contribute to a phenotype.
