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Chunk #29 — Materials and Methods — Whole-cell patch-clamp electrophysiology

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A discrete alcohol pocket involved in GIRK channel activation.
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followed by Bonferroni multiple comparison post-hoc test. To determine the IC50, the inhibition curves were fit with the Hill equation y=1/(1+[x/c]^b), where y is fraction current remaining, x is the concentration of alcohol, b is the Hill coefficient and c is the IC50, the concentration of alcohol that produces 50% inhibition48.