Mice were given a single oral gavage treatment of Wy-14,643 (Cayman Chemical Company, Ann Arbor, MI) at 50mg/kg and sacrificed 48 hours thereafter. Wy-14,643 was dissolved in DMSO and added to olive oil (Sigma-Aldrich, St. Louis, MO) to generate a 20% DMSO-WY/olive oil solution. Control mice were similarly treated with the 20% DMSO/olive oil solvent control.
