manual segmentation of the olfactory bulb (Figure 7) and the basal forebrain/septum (Figure 8) confirmed significant reductions in volume in adult mice that received adolescent binge ethanol treatment. The mouse olfactory bulb is a large frontal brain region representing over 5% of mouse brain volume that is embedded in the skull making automated skull striping and segmentation difficult. Olfactory bulb volume was reduced 6.5% by automated analysis (Table 3) and 7.8% (Figure 7, **p<0.005) by manual segmentation. Overlays show overall olfactory bulb reductions (Figure 7), consistent with binge ethanol treatment induced adolescent olfactory bulb and cortex necrosis (Crews et al., 2000a) and inhibition of adolescent frontal neurogenesis(Crews et al., 2006) found in rat studies.
