SVZ of the CNP- and WT mice, mouse brains were electroporated using the ECM 830 BTX electroporator (Harvard Apparatus, Holliston, MA). Each electroporation result was reproduced in multiple brains derived from at least three separate litters. SVZ NPC cell depletion using Ara-C (2%, Sigma) in vehicle (0.9% saline) or vehicle alone was performed as previously described3. EGF (100nm/ul, Upstate) in vehicle (0.9% saline), or vehicle alonewas infused into the LV of adult GFAP-GFP and WT mice (infusion coordinates: anterioposterior, 0; lateral, 1.1; dorsoventral, 1.5 mm medial to lateral relative to bregma) for 5 days using micro-osmotic pumps (Alzet, model 1007). Brains were then processed for FACS-purification, cell culture or histology. At least 3 different brains for each strain and each experimental condition were analyzed and counted. Cell counting was performed blindly and tissue sections were matched across samples. The analysis of the SVZ was performed at different anterior-posterior and dorso-ventral levels of the lateral ventricle. Statistical analysis was performed by unpaired t-test.
