To inject virus into eyes, adult mice were anesthetized with ketamine/xylazine by intraperitoneal injection. A 30½G needle was used to make a small hole in the temporal eye, below the cornea, and 1.5 μL of rAAV virus was injected into the vitreous humor with a Hamilton syringe and 33G blunt-ended needle. Animals were euthanized and retinas were dissected 4-6 weeks following injection. For cerebellar virus infection, P1-P2 mice were anesthetized with ice and a small puncture was made into caudal-medial position of one cortical lobe; 1.5 μL of rAAV2/9-GFP; mCherry virus was injected with a Hamilton syringe and 33G blunt-ended needle. Mice were analyzed 12 to 35 days post-infection.
