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Chunk #8 — Materials and methods — Statistical analysis

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Brain region- and sex-specific alterations in DAMGO-stimulated [(35) S]GTPγS binding in mice with Oprm1 A112G.
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Two-way ANOVA was used to determine statistical significant differences between groups, with sex and genetic factor as main factors. For comparison of two groups within one factor, two-tailed Student’s t test was performed. *p<0.05, **p<0.01 was the level of significance in all statistical analyses.