A single, continuous acute exposure of C. elegans to 400 mM exogenous ethanol yields an internal concentration of 40–50 mM (Alaimo et al., 2012) (~200 mg/dL, within the range observed in humans after heavy drinking (Bond et al., 2010)). A concentration dependent slowing of locomotion at 10 minutes exposure (measuring initial sensitivity) is followed at ~30 minutes by an increase in speed of locomotion (measuring acute functional tolerance, AFT) (Davies et al., 2003; Davies et al., 2004) despite an increase in the internal tissue concentration of ethanol (Alaimo et al., 2012). Both measures can be independently affected by the loss of individual genes (Davies et al., 2003; Davies et al., 2004; Kapfhamer et al., 2008; Bettinger et al., 2012; Bhandari et al., 2012; Mathies et al., 2015).
