Chunk #17 — Material and Methods — Statistics

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Varenicline, a partial agonist at neuronal nicotinic acetylcholine receptors, reduces nicotine-induced increases in 20% ethanol operant self-administration in Sprague-Dawley rats.

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Statistical analysis was performed using SigmaStat version 3.5 (Systat Software, San Jose, CA). The behavioral data were analyzed using t-test and two-way ANOVA where appropriate, followed by Newman-Keuls post hoc analysis when a significant overall main effect was found (p < 0.05). The BEC data was plotted against the ethanol intake (g/kg/30min) measured prior to blood collection and analyzed by linear regression.