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Chunk #4 — METHODS — Behavioral testing

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Social isolation and chronic handling alter endocannabinoid signaling and behavioral reactivity to context in adult rats.
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The day before behavioral testing, rats were brought into the testing room for 30 min within their own cages to habituate them to the testing room. Behavioral tests were conducted during the light phase of the light:dark cycle between 10:00 AM to 4:00 PM. Between tests, behavioral equipment was wiped cleaned with a 5% acetic acid solution that was allowed to dry before reusing equipment.