The Affymetrix GeneChip miRNA 3.0 microarray contains probesets to measure the expression level of 1733 human mature miRNAs from miRBase v.17 (www.mirbase.org), the primary repository for annotated miRNAs [90]. Total RNA (500ng) from each specimen was labeled using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix). Each RNA sample was spiked with five different oligonucleotides (as positive endogenous controls) to assess the efficiency of the labeling reaction. The RNA samples were subjected to a brief Poly(A) tailing reaction followed by ligation of a biotinylated signal molecule. Each labeled sample was then hybridized onto a GeneChip® miRNA 3.0 Array, and scanned on a GeneChip® Scanner 3000 7G as described above. The microarray data was submitted to the NCBI GEO archive and are available under GSE62699.
