For all experimental conditions (four controls and three schizophrenia iPSC lines, hESC H9 and HUES8) 4–5 organoids for each line were cryosectioned at 30 μm thickness and processed as described in the Supplementary Methods. The antibodies used are listed in Supplementary Table 1. The control and experimental organoid sections were immunostained at the same time using identical protocols28,43,44. Each stain was performed independently four times.
