The field will continue to develop rapidly as more studies are completed and there is much scope for new methodology. In particular, combinations of the current methods may prove to be fruitful -- for example, including combined evidence tests within a two-stage design. There is no best method for all studies, because of their differing properties and aims, but this review has identified some of the questions that should guide the choice of analysis method. Another important area for development, which has not been discussed here, will be the incorporation of evidence from several sources, including association studies, gene ontology annotation, information from model organisms and structural bioinformatics, to give a holistic appraisal of the effects of genetic variation.
