controls, after which analysis proceeded as described above. To examine the robustness of this approach, we also analysed the data using a two-way ANOVA of raw, non-normalized optical densities, with time (0, 3, 7, 21 d) and treatment (alcohol or air) as factors, followed by post-hoc comparison of the respective alcohol-exposed group and its corresponding control at each time-point using Newman–Keuls test. The results of the two approaches were highly concordant. Both sets are provided in the Results section, and the normalized data are shown in Fig. 4a. In all analyses, p<0.05 was considered to be significant.
