Primary genotyping was performed on the Illumina GoldenGate platform, with 975 candidate gene single-nucleotide polymorphisms (SNPs) and 216 ancestry informative marker SNPs. In addition, 89 complementary SNPs were genotyped using ABI Taqman and SNPlex methods. Genotyping call rates for all samples were ⩾94% (mean=99.8% median=99.8%). Genotyping call rates for all SNPs were ⩾97% (mean=99.8%, median=99.9%). SNPs were tested for Hardy–Weinberg equilibrium. In total, 12 SNPs were significantly out of HWE (p<0.0001). None of these were associated with any phenotypes tested here. Multidimensional scaling analysis of the ancestry informative markers identified four subjects that did not cluster with the subjects of European ancestry. These subjects were excluded from the analysis.
