Statistical analysis was performed using Graphpad Prism 7 software. Comparisons involving more than two groups utilized one-way ANOVA followed by Tukey’s post hoc test and corrected p-values for multiple comparisons were reported. Comparison’s with more than two groups and comparing to a control or vehicle group utilized one-way ANOVA followed by Dunnett’s post hoc test with corrected p-values for multiple comparisons reported. Two-Way ANOVA were followed by Sidak’s multiple-comparison post-hoc test Comparisons of two groups utilized two-tailed Students t-test. All differences were considered significantly different when p<0.05. Statistical analysis for RNA-sequencing is detailed above and all further statistical analysis details are reported in the figure legends.
